FEULGEN NUCLEAL REACTION
نویسندگان
چکیده
منابع مشابه
A Chromatographic Study of Hydrolysis in the Feulgen Nucleal Reaction
In a study on Feulgen hydrolysis of frozen-dried alcohol-fixed lily anthers, a chromatographic technique was developed to analyze the acid hydrolysate for some of the degradation products of nucleic acid. Hydrolysis was accomplished by 10 per cent perchloric acid at 20 degrees C., and a typical hydrolysis time-Feulgen intensity curve was obtained, with maximum staining occurring at 19 hours. Mi...
متن کاملFEULGEN NUCLEAL REACTION I. QUANTITATIVE EXTRACTION OF FUCHSIN FROM FEULGEN-STAINED NUCL*OPROTEII~ BY TOSISUKE HIRAOKA, D.Sc
Recent advances in cytochemistry have revealed that two types of nucleic acids play important roles in the cell (for references, 1 and 2). Whereas these two types of nucleic acids, deoxypentose nucleic acid (DNA) and pentose nucleic acid (PNA), have similar ultraviolet absorption properties, they are distinguished from each other by certain color reactions based on their constituent sugars. Amo...
متن کاملA Chromatographic Study of Hydrolysis in the Feulgen Nucleal Reaction* by Philip
Acid hydrolysis of tissue has long been recognized as a necessary part of the Feulgen method for demonstrating the presence of DNA (deoxyribonucleic acid) in histological preparations (Feulgen and Rossenbeck, 1924). When attempts were made to use the method for the quantitative determination of DNA (Pollister and Ris, 1947; Di Stefano, 1948; Ris and Mirsky, 1949; Swift, 1950 a), the role of hyd...
متن کاملFEULGEN NUCLEAL REACTION I. QUANTITATIVE EXTRACTION OF FUCHSIN FROM FEULGEN-STAINED NUCL*OPROTEII~ BY TOSISUKE HIRAOKA, D.Sc
Recent advances in cytochemistry have revealed that two types of nucleic acids play important roles in the cell (for references, 1 and 2). Whereas these two types of nucleic acids, deoxypentose nucleic acid (DNA) and pentose nucleic acid (PNA), have similar ultraviolet absorption properties, they are distinguished from each other by certain color reactions based on their constituent sugars. Amo...
متن کاملQuantitative Cytochemical Determination of Desoxyribonucleic Acid with the Feulgen Nucleal Reaction
The possibility of using the Feulgen nucleal reaction for a quantitative cytochemical estimation of desoxyribonucleic acid (DNA) was investigated. The intensity of the reaction in nuclei was determined by absorption measurements with the microscope. The accuracy of such measurements was tested by comparison with measurements on the same material with a Beckman spectrophotometer. The values obta...
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ژورنال
عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology
سال: 1957
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.3.4.525